kelolalaut.com - Microbiological methods represent the possible presence of bacteria or organisms by enumerating the total numbers of microorganisms, or presence or absence of specific microorganisms such as  Salmonella sp,  E. Coli, Faecal Streptococci, Staphylococcus aureus etc, that can give an impression of microbiological quality, potential indicator of spoilage, indicator of the sanitary conditions, indicator of level of Good Manufacturing Practices (GMP) during processing. Microbiological methods involve growing microorganisms in specific nutrient agar media or in special media that support only particular microorganisms. Here are some parameters that can be used :

- Total Aerobic Count (TAC) and Standard Plant Count (SPC). The total count represents the total number of bacteria that are capable of forming visible colonies on a culture media at a given temperature. Common plate count agar is the substrate used for determination of total count.

- Direct epiflourescence filter technique (DEFT). Microscopic examination of foods is a rapid way of estimating bacterial levels. In this method, cells are stained with acridine orange and then detected by flourescence microscope. Different peptone-rich substrates containing ferric citrate have been used for detection of H2S producing bacteria.